Figure 5. (-)-Xestospongin C and ryanodine down regulate Ca2+ responses to TCR agonist

A and B, average [Ca2+]i responses to 20 μM PHA, applied in nominally Ca2+-free extracellular solution, and readdition of 0.5 mM extracellular Ca2+ in cells preincubated with μ10 M (-)-XeC (A; red trace) or 400 μM Ry (B; blue trace), or vehicle alone (black traces, Control). Each trace represents an average of 20 traces recorded from the individual cells.