James Trimmer, Ph.D.
Professor
1163 Life Sciences
Davis Campus
530-754-6075
e-mail

For more information, visit the Trimmer Laboratory website.

As neuroscience enters the post-genomic era, a major goal is the translation of genomic sequence information into a molecular understanding of the mechanisms of neuronal information processing and transfer. My laboratory’s research focuses on protein function, biochemical pathways and networks of protein-protein interactions regulating intra- and inter-cellular signaling in mammalian neurons. In particular, we are interested in dynamic regulation of voltage-sensitive ion channel abundance, localization and function through reversible protein phosphorylation. These proteins determine the intrinsic electrical properties of neurons and how these cells respond to external stimuli, integrate the encoded information and generate an appropriate response. Modern proteomic techniques have allowed for insights into protein networks, and post-translational modifications, that provide for both the generation and maintenance of complex cellular functions, but also their dynamic regulation that underlies functional plasticity. Our studies are aimed at a molecular understanding of how neuronal ion channels generate and maintain the fidelity of neuronal signaling, and how these processes can be dynamically regulated to generate neuronal plasticity. Such information is necessary for an increased understanding of not only the normal functional plasticity of neurons, but also in understanding of disease states where neuronal function is altered and effects of acute external insults such as ischemia and drugs of abuse, and represent a key step towards the development of therapeutics that can address these and other psychiatric and neurological disorders. Moreover, these studies are representative of approaches that would prove advantageous to studies on other neuronal signaling proteins. To better translate findings from genome-based studies, we have also established the UC Davis/NIH NeuroMab facility, to use information on proteins encoded in the human and other genomes to generate monoclonal antibodies for use in the research community.

See: An updated list of current publications on PubMed »

Kirmiz, M., Palacio, S., Thapa, P., King, A. N., Sack, J. T. and J. S. Trimmer. (2018). Remodeling Neuronal ER-PM Junctions is a Conserved Nonconducting Function of Kv2 Plasma Membrane Ion Channels. Mol. Biol. Cell 29: 2410-2432. PMID: 30091655.

Kirmiz, M., Vierra, N. C., Palacio, S., and J. S. Trimmer. (2018). Identification of VAPA and VAPB as Kv2 Channel-Interacting Proteins Defining Endoplasmic Reticulum-Plasma Membrane Junctions in Mammalian Brain Neurons. J. Neurosci. 38: 7562-7584. PMID: 30012696.

Taussig, M. J., Fonseca, C., and J. S. Trimmer. (2018). Antibody Validation: A View from the Mountains. New Biotech. 45: 1-8. PMID: 30086383.

Baba, K., Yoshida, W., Toriyama, M., Shimada, T., Manning, C. F., Kohno, K., Trimmer, J. S., Watanabe, R., and N. Inagaki. (2018). Gradient-Reading and Mechano-Effector Machinery for Netrin-1–Induced Axon Guidance. eLife 7. pii: e34593. PMID: 30082022.

Simhal, A. K., Gong, B., Trimmer, J. S., Weinberg, R. J., J. Smith, S. J., Sapiro, G., and K. D. Micheva. (2018). Automated Antibody Characterization and Screening for Array Tomography via Probabilistic Synapse Detection. Front. Neuroanat. 12:51, doi: 10.3389/fnana.2018.00051. PMID: 30065633.

Bradbury, A. R. M.,Trinklein, N. D., Thie, H., Wilkinson, I. C., Tandon, A. K., Anderson, S., Bladen, C. L., Jones, S., Force Aldred, S., Bestagno, M., Burrone, O., Maynard, J., Ferrara, F., Trimmer, J. S., Görnemann, J., Glanville, J., Wolf, P., Frenzel, A., Wong. J., Yu, X., Eng, H.-Y., Lane, D, Lefranc, M.-P., Clark, M., & Dübel, S. (2018). When Monoclonal Antibodies are not Monospecific: Hybridomas Frequently Express Additional Functional Variable Regions. MAbs. 27:1-19. PMID: 29485921.

Riquelme, D., Silva, I., Philp, A. M., Huidobro-Toro, J. P., Cerda, O., Trimmer J. S., and E. Leiva-Salcedo. (2018). Subcellular Localization and Activity of TRPM4 in Medial Prefrontal Cortex Layer 2/3. Front. Cell. Neurosci. 12:12, doi: 10.3389/fncel.2018.00012. PMID: 29440991.

Bishop, H. I., Cobb, M. M., Kirmiz, M., Parajuli, L., Mandikian, D., Philp, A. M., Melnik, M., Kuja-Panula, J., Rauvala, H., Shigemoto, R., Murray, K. D., and J. S. Trimmer. (2018). Kv2 Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. Front. Mol. Neurosci. 11:1. DOI: 10.3389/fnmol.2018.00001. PMID: 29403353.

Jensen, C. S., Watanabe, S., Stas, J. I., Klaphaak, J., Schmitt, N., Olesen, S.-P., Trimmer, J. S., Rasmussen, H. B., and H. Misonou. (2017). Trafficking of Kv2.1 Channels to the Axon Initial Segment by a Novel Non-Conventional Secretory Pathway. J. Neurosci. 37:11523-11536. PMID: 29042434.

Speca, D. J., Trimmer, J. S., Peterson, A. S., and E. Diaz. (2017). Whole Exome Sequencing Reveals a Functional Mutation in the GAIN Domain of the Bai2 Receptor Underlying a Forward Mutagenesis Hyperactivity QTL. Mammalian Genome 28:465-475. PMID: 28894906.

Palacio, S., Chevaleyre, V., Brann, D. H., Murray, K. D., Piskorowski, R. A., and J. S. Trimmer. (2017). Heterogeneity in Kv2 Channel Expression Shapes Action Potential Characteristics and Firing Patterns in CA1 Versus CA2 Hippocampal Pyramidal Neurons. eNeuro 4(4). pii: ENEURO.0267-17.2017. PMID: 28856240.

Kirk, L. M., Ti, S. W., Bishop, H. I., Orozco-Llamas, M., Pham, M., Trimmer, J. S., Díaz, E. (2016). Distribution of the SynDIG4/Proline-Rich Transmembrane Protein 1 in Rat Brain. J. Comp. Neurol. 524:2266-2280. PMID: 26660156.

Alexander, S. P., Kelly, E., Marrion, N., Peters, J. A., Benson, H. E., Faccenda, E., Pawson, A. J., Sharman, J. L., Southan, C., Buneman, O. P., Catterall, W. A., Cidlowski, J. A., Davenport, A. P., Fabbro, D., Fan, G., McGrath, J. C., Spedding, M., Davies, J. A.; CGTP Collaborators. (2015). The Concise Guide to PHARMACOLOGY 2015/16: Overview. Br. J. Pharmacol. 172:5729-5743. PMID: 26650438.

Gong, B., Murray, K. D., and J. S. Trimmer. (2015). Developing High-Quality Mouse Monoclonal Antibodies for Neuroscience Research – Approaches, Perspectives and Opportunities. New Biotech. 33:551-564. PMID: 26644354.

Cobb, M. M., Austin, D. C., Sack, J. T., and J. S. Trimmer. (2015). Cell Cycle-Dependent Changes in Localization and Phosphorylation of the Plasma Membrane Kv2.1 K+ Channel Impact Endoplasmic Reticulum Membrane Contact Sites in COS-1 Cells. J. Biol. Chem. 290:29189-29201. PMID: 26442584.

Bishop, H. I., Guan, D., Bocksteins, E., Parajuli, L., Murray, K. D., Cobb, M. M., Misonou, H., Zito, K., Foehring, R. C., and J. S. Trimmer. (2015). Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons. J. Neurosci. 35:14922-14942. PMID: 26538660.

Thiffault, I., Speca, D. J., Austin, D. C., Cobb, M. M., Eum, K. S., Safina, N. P., Grote, L., Farrow, E. G., Miller, N., Soden, S., Kingsmore, S. F., Trimmer, J. S., Saunders, C. J., and J. T. Sack. (2015) A Novel Epileptic Encephalopathy Mutation in KCNB1 Disrupts Kv2.1 Ion Selectivity, Expression, and Localization. J. Gen. Physiol. 146:399-410. PMID: 26503721.

Cerda, O., Leiva-Salcedo, E., Park, K.-S., Cáceres, M., Romeroa, A., Varela, D., Trimmer, J. S., and A. Stutzinband. (2015). Casein Kinase-Mediated Phosphorylation of Serine 839 Is Necessary for Basolateral Localization of the Ca2+-Activated Non-Selective Cation Channel TRPM4. Pflugers Arch. 467: 1723-1732. PMID: 25231975.

Jan, L. Y., Attali, B., Nerbonne, J., Sanguinetti, M. C., Trimmer, J. S., Chandy, K. G., Grissmer, S., Gutman, G. A., Lazdunski, M., Mckinnon, D., Pardo, L. A., Robertson, G. A., Rudy, B., Stühmer, W., and X. Wang. (2015).Voltage-Gated Potassium Channels. IUPHAR/BPS Guide to PHARMACOLOGY.

Cáceres, M., Ortiz, L., Romero, A., Recabarren, T., Colombo, A., Leiva-Salcedo, E., Varela, D., Rivas, J., Silva, I., Campusano, C., Almarza, O., Simón, F., Toledo, H., Park, K.-S., Trimmer, J. S., and O. Cerda. (2015). TRPM4 is a Novel Component of the Adhesome Required for Focal Adhesion Disassembly, Migration and Contractility. PLoS ONE 10:e0130540. PMID: 26110647.

Hirono, M., Ogawa, Y., Misono, K., Zollinger, D. R., Trimmer, J. S., Rasband, M. N., and H. Misonou. (2015). BK Channels Localize to the Paranodal Junction and Regulate Action Potentials in Myelinated Axons of Cerebellar Purkinje Cells. J. Neurosci. 35:7082-7094. PMID: 25948259.

Trimmer, J. S., and H. Misonou. (2015). Ion Channel Phosphorylation. (Chapter 36, pages 531-544). In The Handbook of Ion Channels. (J. Zheng and M. C. Trudeau, Editors). CRC Press, Boca Raton, FL.

Hsiao, J. J., Ng, B. H., Smits, M. M., Wang, J., Jasavala, R. J., Martinez, H. D., Lee, J. A., Alston, J. J., Misonou, H., Trimmer, J. S., and M. E. Wright. (2015). Androgen Receptor and Chemokine Receptors 4 and 7 Form a Signaling Axis to Regulate CXCL12-Dependent Cellular Motility. BMC Cancer 15:204. PMID: 25884570.

Trimmer, J. S. (2015). Subcellular Localization of K+ Channels in Mammalian Brain Neurons: Remarkable Precision in the Midst of Extraordinary Complexity. Neuron 85: 238-256. PMID: 25611506

  • Established Investigatorship, American Heart Association
  • National Institute of Neurological Disorders and Stroke, National Institutes of Health, Jacob Javits Neuroscience Investigator (MERIT) Award
  • UC Davis Graduate Student Association Award for Excellence in Service to Graduate Students
  • NPB 107, Cell Signaling in Health and Disease
  • NPB 110A Foundations: From Molecules to Individuals
  • National Institutes of Health