John Voss, Ph.D.

Professor
4110 Tupper Hall
Davis Campus
Ph: 530-754-7583
e-mail
Lab Website

Research Interests

My research interests are in observing molecular switching in proteins, as well as protein folding and assembly. This work is carried out in large part through the use electron paramagnetic resonance (EPR — a.k.a. ESR) spectroscopy of site-directed spin labels. We use this approach to address problems in several biological systems, including those related to cardiovascular and neurodegenerative diseases.

Graduate Group Affiliations

See: An updated list of current publications

Meruyert Imanbekova M, Suarasan S, Rojalin T, Mizenko RR, Hilt S, Mathur M, Lepine P, Nicouleau M, Mohamed N, Durcan TM, Carney RP, Voss JC, Wachsmann-Hogiu S. Identification of amyloid beta in small extracellular vesicles via Raman spectroscopy. Nanoscale Advances, 2021, in press, DOI: 10.1039/d1na00330e.

Waldie S, Sebastiani F, Moulin M, Del Giudice R, Paracini N, Roosen-Runge F, Gerelli Y, Prevost S, Voss JC, Darwish TA, Yepuri N, Pichler H, Maric S, Forsyth VT, Haertlein M, Cárdenas M. ApoE and ApoE Nascent-Like HDL Particles at Model Cellular Membranes: Effect of Protein Isoform and Membrane Composition. Front Chem. 2021 Apr 29;9:630152. doi: 10.3389/fchem.2021.630152. PMID: 33996741.

Suarasan S, Liu J, Imanbekova M, Rojalin T, Hilt S, Voss JC and Wachsmann-Hogiu S. Superhydrophobic bowl-like SERS substrate patterned from CMOS sensors for extracellular vesicles characterization. J. Mater. Chem. B, 2020,8, 8845-8852.

Bansal S, Su WC, Budamagunta M, Xiao W, Ajena Y, Liu R, Voss JC, Carney RP, Parikh AN, Lam KS. Discovery and mechanistic characterization of a structurally-unique membrane active peptide. Biochim Biophys Acta Biomembr. 2020 Oct 1;1862(10):183394. doi: 10.1016/j.bbamem.2020.183394. Epub 2020 Jun 18. PMID:32562695.

de Oliveira MAS, Hilt S, Chang CW, Lee C, Voss JC, Chan JW. Surface-enhanced Raman scattering sensing platform for detecting amyloid-β peptide interaction with an aggregation inhibitor. Appl Opt. 2020 Sep 1;59(25):7490-7495. doi: 10.1364/AO.399307. PMID: 32902446

Alshehab M, Budamagunta MS, Voss JC and Nitin N. Real-time measurements of milk fat globule membrane modulation during simulated intestinal digestion using electron paramagnetic resonance spectroscopy. Colloids Surf B Biointerfaces, 2019. 184: p. 110511.

Gae, DD, Budamagunta, MS, Hess, JF, McCarrick, RM, Lorigan, GA, FitzGerald, PG and Voss, JC. Completion of the Vimentin Rod Domain Structure Using Experimental Restraints: A New Tool for Exploring Intermediate Filament Assembly and Mutations. Structure, 2019; 27, 1–14.

Nyunt T, Britton M, Wanichthanarak K, Budamagunta M, Voss JC, Wilson DW, Rutledge JC, Aung HH. Mitochondrial oxidative stress-induced transcript variants of ATF3 mediate lipotoxic brain microvascular injury. Free Radical Biology and Medicine, 2019; 143:25-46. doi: 10.1016/j.freeradbiomed.2019.07.024.

Hilt N, Altman R, Kálai T, Maezawa I, Qizhi Gong Q, Wachsmann-Hogiu S, Jin L-J, Voss JC. A Bifunctional Anti-Amyloid Blocks Oxidative Stress and the Accumulation of Intraneuronal Amyloid-Beta. Molecules, 2018, 23(8). pii: E2010. doi: 10.3390/molecules23082010.

Budamagunta MS, Guo F, Sun N, Shibata B, FitzGerald PG, Voss JC, Hess JF. Production of recombinant human tektin 1, 2, and 4 and in vitro assembly of human tektin 1. Cytoskeleton, 2018; 75(1):3-11. doi: 10.1002/cm.21418. PMID: 29108134

Hilt S, Rojalin T, Viitala T, Koivuniemi A, Bunker A, Wachsmann-Hogiu S, Kálai T, Hideg K, Yliperttula M, Voss J. Oligomerization Alters Binding Affinity between Amyloid Beta and a Modulator of Peptide Aggregation. J Physical Chemistry C. 2017 October 25; 121(43):23974-23987.

Shuai Z, Wang J, Badamagunta M, Choi J, Yang G, Zhang W, Kenny TP, Guggenheim K, Kurth MJ, Ansari AA, Voss J, Coppel RL, Invernizzi P, Leung PS, Gershwin ME. The Fingerprint of Antimitochondrial Antibodies and the Etiology of Primary Biliary Cholangitis. Hepatology. 2017, 65(5):1670-1682. doi: 10.1002/hep.29059. PubMed PMID: 28100006.

Hilt S, Tang T, Walton JH, Budamagunta M, Maezawa I, Kálai T, Hideg K, Singh V, Wulff H, Gong Q, Jin LW, Louie A, Voss JC. A Metal-Free Method for Producing MRI Contrast at Amyloid-β. J Alzheimers Dis. 2017;55(4):1667-1681. doi:10.3233/JAD-160279. PubMed PMID: 27911291.

Petrlova J, Hilt S, Budamagunta M, Domingo-Espín J, Voss JC, Lagerstedt JO. Molecular crowding impacts the structure of apolipoprotein A-I with potential implications on in vivo metabolism and function. Biopolymers. 2016 Oct;105(10):683-92. doi: 10.1002/bip.22865. PubMed PMID: 27122373.

Aung HH, Altman R, Nyunt T, Kim J, Nuthikattu S, Budamagunta M, Voss JC, Wilson D, Rutledge JC, Villablanca AC. Lipotoxic brain microvascular injury is mediated by activating transcription factor 3-dependent inflammatory and oxidative stress pathways. J Lipid Res. 2016 Jun;57(6):955-68. doi:10.1194/jlr.M061853. PubMed PMID: 27087439.

Chernyatina AA, Hess JF, Guzenko D, Voss JC, Strelkov SV. How to Study Intermediate Filaments in Atomic Detail. Methods Enzymol. 2016;568:3-33. doi: 10.1016/bs.mie.2015.09.024.

Hong H-S, Maezawa I, Petrlova J, Zhao XY, Voss JC, Jin LW. Tomoregulin (TMEFF2) Binds Alzheimer's Disease Amyloid-beta (A beta) Oligomer and A beta PP and Protects Neurons from A beta-Induced Toxicity. J. Alzheimer’s Disease, 2015; 48. 731-743. DOI:10.3233/JAD-150318.

Altman, R., S. Ly, S. Hilt, J. Petrlova, I. Maezawa, T. Kalai, K. Hideg, L.W. Jin, T.A. Laurence, and J.C. Voss, Protective spin-labeled fluorenes maintain amyloid beta peptide in small oligomers and limit transitions in secondary structure. Biochim Biophys Acta, 2015. 1854(12): p. 1860-1870.

Zeno WF, Hilt S, Risbud SH, Voss JC, Longo ML. Spectroscopic Characterization of Structural Changes in Membrane Scaffold Proteins Entrapped within Mesoporous Silica Gel Monoliths. ACS Applied Materials & Interfaces. 2015, 7, 8640-9.

Zeno WF, Hilt S, Aravagiri KK, Risbud SH, Voss JC, Parikh AN, Longo ML. Analysis of lipid phase behavior and protein conformational changes in nanolipoprotein particles upon entrapment in sol-gel-derived silica. Langmuir. 2014 Aug 19;30(32):9780-8. doi: 10.1021/la5025058.

Petrlova J, Dalla-Riva J, Mörgelin M, Lindahl M, Krupinska E, Stenkula KG, Voss JC, Lagerstedt JO. Secondary structure changes in ApoA-I Milano (R173C) are not accompanied by a decrease in protein stability or solubility. PLoS One. 2014 Apr 22;9(4):e96150. doi: 10.1371/journal.pone.0096150.

Kálai T, Altman R, Maezawa I, Balog M, Morisseau C, Petrlova J, Hammock BD, Jin LW, Trudell J, Voss JC, Hideg. Synthesis and functional survey of new Tacrine analogs modified with nitroxides or their precursors. Eur J Med Chem, 2014; 77, 343-50.

Kemmerer S, Voss JC, Faller R.. Molecular dynamics simulation of dipalmitoylphosphatidylcholine modified with a MTSL nitroxide spin label in a lipid membrane. Biochim Biophys Acta. 2013, 28:2770-7.

Oda MN, Budamagunta MS, Geier EG, Chandradas SH, Shao B, Heinecke JW, Voss JC, and Cavigiolio G. Conservation of apolipoprotein A-I’s central domain structural elements upon lipid association on different HDL subclasses. Biochemistry, 2013; 52, 6766-78.

Wang J, Budamagunta MS, Voss JC, Kurth MJ, Lam KS, Lu L, Kenny TP, Bowlus C, Kikuchi K, Coppel RL, Ansari AA, Gershwin ME, Leung PS. Antimitochondrial Antibody Recognition and Structural Integrity of the Inner Lipoyl Domain of the E2 Subunit of Pyruvate Dehydrogenase Complex. J Immunol. 2013, 191, 2126-33.

Myers WK, Xu X, Li C, Lagerstedt JO, Budamagunta MS, Voss JC, Britt RD, Ames JB. Double Electron-Electron Resonance Probes Ca2+-induced Conformational Changes and Dimerization of Recoverin. Biochemistry. 2013, 52, 5800-8.

Oda MN, Budamagunta MS, Borja MS, Petrlova J, Voss JC, Lagerstedt JO. The secondary structure of apolipoprotein A-I on 9.6-nm reconstituted high-density lipoprotein determined by EPR spectroscopy. FEBS J. 2013; 280: 3416-24. doi: 10.1111/febs.12334.

Ly S, Altman R, Petrlova J, Lin Y, Hilt S, Huser T, Laurence TA, and Voss JC. Binding of apolipoprotein E inhibits the oligomer growth of amyloid beta in solution as determined by fluorescence cross correlation spectroscopy. J Biol Chem. 2013; 288, 11628-35.

Acar S, Carlson D, Budamagunta M, Yarov-Yarovoy V, Correia JJ, Ninonuevo M, Jia M, Tao L, Leary JL, Voss JC, Evans J, Scholey JM. The Bipolar Assembly Domain of the Mitotic Motor, Kinesin-5. Nature Commun. 2013, 4, 1343.

Lagerstedt JO, Petrlova J, Hilt S, Marek A, Chung Y, Sriram R, Budamagunta MS, Desreux JF, Thonon D, Jue T, Smirnov AI, and Voss JC. EPR assessment of protein sites for incorporation of Gd(III) MRI contrast labels. Contrast Media Mol Imaging, 2013, 8, 252-264.

Hess JF, Budamagunta MS, Aziz A, Fitzgerald PG, Voss JC. Electron paramagnetic resonance analysis of the vimentin tail domain reveals points of order in a largely disordered region and conformational adaptation upon filament assembly. Protein Sci. 2013, 22,47-55.

den Hartigh LJ, Altman R, Hutchinson R, Petrlova J, Budamagunta MS, Tetali SD, Lagerstedt JO, Voss JC, Rutledge JC., Postprandial apoE isoform and conformational changes associated with VLDL lipolysis products modulate monocyte inflammation., PLoS One. 2012;7(11):e50513. doi: 10.1371/journal.pone.0050513. Epub 2012 Nov 28.

Li Y, Budamagunta MS, Luo J, Xiao W, Voss JC, Lam KS., Probing of the assembly structure and dynamics within nanoparticles during interaction with blood proteins., ACS Nano. 2012 Nov 27;6(11):9485-95. doi: 10.1021/nn302317j. Epub 2012 Oct 30.

Aziz A, Hess JF, Budamagunta MS, Voss JC, Kuzin AP, Huang YJ, Xiao R, Montelione GT, FitzGerald PG, Hunt JF., The structure of vimentin linker 1 and rod 1B domains characterized by site-directed spin-labeling electron paramagnetic resonance (SDSL-EPR) and X-ray crystallography., J Biol Chem. 2012 Aug 17;287(34):28349-61. doi: 10.1074/jbc.M111.334011. Epub 2012 Jun 26.

Petrlova J, Kálai T, Maezawa I, Altman R, Harishchandra G, Hong HS, Bricarello DA, Parikh AN, Lorigan GA, Jin LW, Hideg K, Voss JC., The influence of spin-labeled fluorene compounds on the assembly and toxicity of the aβ peptide., PLoS One. 2012;7(4):e35443. doi: 10.1371/journal.pone.0035443. Epub 2012 Apr 30.

Lagerstedt JO, Budamagunta MS, Liu GS, DeValle NC, Voss JC, Oda MN., The "beta-clasp" model of apolipoprotein A-I--a lipid-free solution structure determined by electron paramagnetic resonance spectroscopy., Biochim Biophys Acta. 2012 Mar;1821(3):448-55. doi: 10.1016/j.bbalip.2011.12.010. Epub 2012 Jan 8.

Chen HK, Liu Z, Meyer-Franke A, Brodbeck J, Miranda RD, McGuire JG, Pleiss MA, Ji ZS, Balestra ME, Walker DW, Xu Q, Jeong DE, Budamagunta MS, Voss JC, Freedman SB, Weisgraber KH, Huang Y, Mahley RW., Small molecule structure correctors abolish detrimental effects of apolipoprotein E4 in cultured neurons., J Biol Chem. 2012 Feb 17;287(8):5253-66. doi: 10.1074/jbc.M111.276162. Epub 2011 Dec 12.

Ly S, Petrlova J, Huser T, Fore S, Gao T, Voss J, Laurence TA., Stoichiometry of Reconst High-Density Lipoproteins in the Hydrated State Determined by Photon Antibunching., Biophys J, 2011; 101: 970-975.

Kálai T, Petrlova J, Balog M, Aung HH, Voss JC, Hideg K., Synthesis and study of 2-amino-7-bromofluorenes modified with nitroxides and their precursors as dual anti-amyloid and antioxidant active compounds., Eur J Med Chem. 2011 Apr;46(4):1348-55. doi: 10.1016/j.ejmech.2011.01.059. Epub 2011 Feb 3.

Petrlova J, Hong HS, Bricarello DA, Harishchandra G, Lorigan GA, Jin LW, Voss JC., A differential association of Apolipoprotein E isoforms with the amyloid-β oligomer in solution., Proteins. 2011 Feb;79(2):402-16. doi: 10.1002/prot.22891.

Lagerstedt JO, Cavigiolio G, Budamagunta MS, Pagani I, Voss JC, Oda MN., Structure of apolipoprotein A-I N terminus on nascent high density lipoproteins., J Biol Chem. 2011 Jan 28;286(4):2966-75. doi: 10.1074/jbc.M110.163097. Epub 2010 Nov 3.

Nygaard EB, Lagerstedt JO, Bjerre G, Shi B, Budamagunta M, Poulsen KA, Meinild S, Rigor RR, Voss JC, Cala PM, Pedersen SF., Structural modeling and electron paramagnetic resonance spectroscopy of the human Na+/H+ exchanger isoform 1, NHE1., J Biol Chem. 2011 Jan 7;286(1):634-48. doi: 10.1074/jbc.M110.159202. Epub 2010 Oct 25.

Tetali SD, Budamagunta MS, Simion C, den Hartigh LJ, Kálai T, Hideg K, Hatters DM, Weisgraber KH, Voss JC, Rutledge JC., VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation., J Lipid Res. 2010 Jun;51(6):1273-83. doi: 10.1194/jlr.M000406. Epub 2009 Dec 3.

Hatters DM, Voss JC, Budamagunta MS, Newhouse YN, Weisgraber KH., Insight on the molecular envelope of lipid-bound apolipoprotein E from electron paramagnetic resonance spectroscopy., J Mol Biol. 2009 Feb 13;386(1):261-71. doi: 10.1016/j.jmb.2008.12.040. Epub 2008 Dec 24.

  • BCM 410A, Medical Biochemistry
  • MCB/BPH 241, Membrane Biology
  • 2000, Scientist Development Award, American Heart Association
  • 2000, Basil O’Connor New Investigator Award, March of Dimes Foundation
  • 1998, Charles E. Culpeper Biomedical Initiative Award