John Alan Payne, Ph.D.
Professor Emeritus
Locations and Contact
Clinical Interests
John A. Payne's research interests involve molecular physiology of membrane transport proteins, with an emphasis on cation-chloride cotransporters. Molecular biological techniques are used to determine distribution and isoform diversity and to identify functional-structural aspects of the transport protein, including ion and inhibitor binding sites, glycosylation sites, and phosphorylation-regulatory sites. Model systems include single cells (e.g. neurons) and renal and intestinal epithelia.
Undergraduate School
B.A., Biology, UC Berkeley, Berkeley CA 1983
Other School
M.S., Physiology, Duke University, Durham NC 1987
Other School
Ph.D., Zoology, University of Florida, Gainesville FL 1991
Fellowship
Cell and Molecular Physiology, Yale University School of Medicine, New Haven CT 1991-1995
Established Investigatorship, American Heart Association, 2002, 2003, 2004, 2005
Young Investigator Award, Epilepsy Foundation of America, 1996
Phi Kappa Phi Honor Society, University of Florida, 1991
Voipio J, Boron WF, Jones SW, Hopfer U, Payne JA, Kaila K. Comment on "Local impermeant anions establish the neuronal chloride concentration". Science. 2014;345:1130.
Kaila K, Price TJ, Payne JA, Puskarjov M, Voipio J. Cation-chloride cotransporters in neuronal development, plasticity and disease. Nat Rev Neurosci. 2014 Oct;15(10):637-54. doi:10.1038/nrn3819. PMID:25234263.
Payne JA. Molecular operation of the cation chloride cotransporters: ion binding and inhibitor interaction. Curr Top Membr. 2012;70:215-37. doi:10.1016/B978-0-12-394316-3.00006-5. PMID:23177987.
Antrobus SP, Lytle C, Payne JA. K+-Cl- cotransporter-2 KCC2 in chicken cardiomyocytes. Am J Physiol Cell Physiol. 2012 Dec 1;303(11):C1180-91. doi:10.1152/ajpcell.00274.2012. Epub 2012 Oct 3. PMID:23034386.
Williams JR, Payne JA. Cation transport by the neuronal K(+)-Cl(-) cotransporter KCC2: thermodynamics and kinetics of alternate transport modes. Am J Physiol Cell Physiol. 2004 Oct;287(4):C919-31. doi:10.1152/ajpcell.00005.2004. Epub 2004 Jun 2. PMID:15175220.
Vu TQ, Payne JA, Copenhagen DR. Localization and developmental expression patterns of the neuronal K-Cl cotransporter (KCC2) in the rat retina. J Neurosci. 2000 Feb 15;20(4):1414-23. doi:10.1523/JNEUROSCI.20-04-01414.2000. PMID:10662832.
Williams JR, Sharp JW, Kumari VG, Wilson M, Payne JA. The neuron-specific K-Cl cotransporter, KCC2. Antibody development and initial characterization of the protein. J Biol Chem. 1999 Apr 30;274(18):12656-64. doi:10.1074/jbc.274.18.12656. PMID:10212246.
Rivera C, Voipio J, Payne JA, Ruusuvuori E, Lahtinen H, Lamsa K, Pirvola U, Saarma M, Kaila K. The K+/Cl- co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation. Nature. 1999 Jan 21;397(6716):251-5. doi:10.1038/16697. PMID:9930699.
Payne JA. Functional characterization of the neuronal-specific K-Cl cotransporter: implications for [K+]o regulation. Am J Physiol. 1997 Nov;273(5):C1516-25. doi:10.1152/ajpcell.1997.273.5.C1516. PMID:9374636.
Payne JA, Stevenson TJ, Donaldson LF. Molecular characterization of a putative K-Cl cotransporter in rat brain. A neuronal-specific isoform. J Biol Chem. 1996 Jul 5;271(27):16245-52. doi:10.1074/jbc.271.27.16245. PMID:8663311.